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Research InterestsDr Andrew Moorhouse | | (Position Details) | | Phone 02 9385 2575 | | Email | | Qualifications BSc, PhD Syd |
| | | School/Unit |
| School of Medical Sciences | |
| | Broad Research Areas | |
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Neuroscience
Physiology
Pharmacology
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| | | | Specific Research Keywords | |
| Structure & Function of Ion Channels
Electrophysiology
Synapse Function and Plasticity
Ligand gated ion channels
Glycine Receptor
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| | | | Research Interests | |
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My main research area is glycine receptors; how their molecular structure relates to their function; their physiological roles at synapses and investigating novel modulators. I also research additional aspects of synaptic transmission and ion channels.
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| | | | ILP Research Interests (Will supervise ILP students) |
| Electrophysiology
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| | | | Project Topics for current Honours Students |
| Students interested in potential research projects should contact me for further discussion. The broad area of research relates to physiology and pharmacology of glycine receptors and/or synapses, and may involve patch-clamp experiments on recombinant ion channels expressed in cell cultures, or experiments on acute brain preparations. | |
| | | | Teaching Interests | |
| My main area of teaching is in the cellular physiology of excitable cells, that is, how cells generate and communicate electrical signals to each other and the basic properties of the membrane transport and synaptic proteins involved. This important aspect of basic cell physiology and cellular neuroscience is taught across a variety of year 1-3 Medicine and Science/Medical Science courses. I am also the current convenor of the Phase 1 Medicine Health Maintenance A course. | |
| | | | Society Memberships & Professional Activities | |
| I am a member of:
- The Australian Neuroscience Society (ANS)
- The Australian Physiological Society (AuPS)
I regularly review grants and papers for a variety of national and International funding bodies and journals. | |
| | | | Funding Sources | |
| ARC, NH&MRC, UNSW FRG, JSPS | |
| | | | Other Postgraduate Research | |
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Students interested in potential research projects should contact me for further discussion. The broad area of research relates to physiology and pharmacology of glycine receptors and/or synapses, and may involve patch-clamp experiments on recombinant ion channels expressed in cell cultures, or experiments on acute brain preparations. | |
| | | | Key works/Publications | |
| A total of twenty-seven scientific papers since 1996, with total citations at 545 (Science Citations Index, September 30., 2008), including 195 citations already for the 15 papers published since 2002.
A selection of papers is below:
Ammala C., Moorhouse A., Gribble F., Ashfield R., Proks P., Smith P.A., Sakura H., Coles B, Ashcroft S.J.H. and Ashcroft F.M.A. (1996) Promiscuous coupling between the sulphonylurea receptor and inwardly rectifying potassium channels. Nature ,379, pp 545-548.
Tucker S.J., Pessia M., Moorhouse A.J., Gribble F., Ashcroft F.M.A., Maylie J. and Adelman J.P. (1996) Heteromeric channel formation and Ca2+-free media reduce the toxic effect of the weaver Kir 3.2 allele. FEBS Letters, 390, pp. 253-257.
Moorhouse A.J., Jacques, P., Barry, P.H. and Schofield, P.R. (1999) The startle disease mutation Q266H, in the second transmembrane domain of the human glycine receptor, impairs channel gating. Molec. Pharmacol., 55 (2), pp 386-395.
Barry, P.H., Schofield, P.R. and Moorhouse, A.J. (1999) Glycine receptors: What gets in and why? Clin. Exp. Pharmacol. Physiol., 26, pp 935-936.
Keramidas, A, Moorhouse, A.J., French, C.R., Schofield, P.R. and Barry, P.H. (2000) M2 pore mutations convert the glycine receptor channel from being anion- to cation-selective. Biophys. J., 79, pp 247-259.
Qu, W., Zhu, X.O., Moorhouse, A.J., Bieri, S., Cunningham, A.M. and Barry, P.H. (2000) Ion permeation and selectivity of wild-type recombinant rat CNG (rOCNC1) channels expressed in HEK293 cells. J. Memb. Biol., 178, 137-150.
Kaur, R., Zhu, X.O., Moorhouse, A.J. and Barry, P.H. (2001) IP3-gated channels and their occurrence relative to CNG channels in the soma and the dendritic knob of rat olfactory receptor neurons. J. Memb. Biol., 181 (2), 91-105.
Keramidas, A, Moorhouse, A.J., Pierce, K.D., Schofield, P.R. and Barry, P.H. (2002) Cation-selective mutations in the M2 domain of the inhibitory glycine-receptor channel reveal determinants of ion charge selectivity. J. Gen. Physiol., 119, 393-410.
Moorhouse, A.J., Keramidas, A, Zaykin, A., Schofield, P.R. and Barry, P.H. (2002) Single-channel analysis of conductance and rectification in cation-selective mutant glycine-receptor channels. J. Gen. Physiol., 119, 411-425.
Akaike, N. and Moorhouse, A.J. (2003) Techniques: applications of the nerve-bouton preparation in neuropharmacology. TIPS., 24, 44-47.
Jeong, H.J., Jang, I.S., Moorhouse, A.J, and Akaike, N. (2003) Activation of presynaptic glycine receptors facilitates glycine release from presynaptic terminals synapsing onto rat spinal sacral dorsal commissural nucleus neurons. J Physiol., 550.2, 373-383.
Keramidas, A., Moorhouse, A.J., Schofield, P.R. and Barry, P.H. (2004) Ligand gated ion channels: Mechanisms underlying ion selectivity. Prog. Biophys. Molec. Biol., 86, 161-204.
Moorhouse, A.J., Li, S., Vickery, R.M., Hill, M.A. and Morley, J.W. (2004). A patch-clamp investigation of membrane currents in a novel mammalian retinal ganglion cell line. Brain Res., 1003, 205-208.
Carland, J.E., Moorhouse, A.J., Barry, P.H., Johnston, G.A. and Chebib, M. (2004) Charged residues at the 2' position of human GABAC rho 1 receptors invert ion selectivity and influence open state probability. J. Biol. Chem., 279, 54153-54160.
Qu, W., Moorhouse, A. J., Chandra, M., Lewis, T. M., Pierce, K. D. and Barry, P. H. (2006). A single P-loop glutamate point mutation to either lysine or arginine switches the cation-anion selectivity of the CNGA2 channel. J. Gen. Physiol., 127, 375-389.
Wake, H., Watanabe, M., Moorhouse, A.J., Kanematsu, K., Horibe, S., Matsukawa, N., Asai, K., Ojika, K., Hirata, M., and Nabekura, J. (2007) Early changes in KCC2 phosphorylation in response to neuronal stress results in functional downregulation. J. Neurosci., 27: 1642-1650.
Park, E.J., Choi, I.S., Cho, J.H., Nakamura, M., Lee, J.J., Lee, M.G., Choi, B.J., Moorhouse, A.J. and Jang, I.S. (2008) Zinc modulation of glycine receptors in acutely isolated rat CA3 neurons. Life Sci., 83: 5-6.
Sugiharto, S., Lewis, T.M., Moorhouse, A.J., Schofield, P.R. and Barry, P.H. (2008) Anion-Cation Permeability Correlates with Hydrated Counter-ion Size in Glycine Receptor Channels. Biophys J. Aug 15. [Epub ahead of print]
Invited Book Chapters
Barry, P.H., Qu, W. and Moorhouse, A.J. (2008). Biophysics of CNG ion channels. In: Sensing with Ion Channels. Ed. B. Martinac, Springer-Verlag, Heidelberg, pp 176-200. | |
| | | | Further Information | |
| http://medicalsciences.med.unsw.edu.au/SOMSWeb.nsf/page/Membrane+Biophysics+Lab | |
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