Immunohistochemistry

HMU routinely performs immunohistochemistry (IHC) and immunofluorescence (IF) on formalin fixed paraffin embedded tissue and fresh frozen tissue sections.
Researchers wishing to use this service, should contact HMU staff to discuss their staining requirements.
Primary and secondary antibodies are usually supplied by the researcher.
Immunohistochemistry staining is either automated (Leica Bond X Immunostainer or Dako Autostainer) or performed manually by our experienced staff.

Bond X Immunostainer

Bond automates: Primary antibody application Secondary antibody application Tertiary antibody step Counterstaining Detection Systems: Bond Intense R Detection Bond Polymer Refine Detection

Dako Autostainer Plus

Uses the EnVision™ FLEX, High pH visualisation system. Secondary mouse and rabbit antibodies Peroxidase-Blocking Reagent EnVision™/HRP DAB+ Chromogen, Substrate Buffer Target Retrieval Solution, pH 9 & pH 6